《米兰app官网版下载官方版》 2018, 30(5): 585-592

DNA与蛋白质的相互作用及其生物学研究方法

孙宇航，王宇祥*

(东北农业大学动物科学技术学院，农业部鸡遗传育种重点实验室，黑龙江省普通高等学校动物遗传育种与繁殖重点实验室，哈尔滨 150030)

摘　要：摘　要：DNA 和蛋白质是构成生物体最为重要的两类生物大分子，两者特异性或非特异性识别及相互作用在整个基因组表达调控过程中发挥着重要的作用，是了解生命活动基本过程的分子基础。为此，从DNA与蛋白质相互作用的概述及其作用形式入手，对目前应用较多的几种分子生物学检测方法，如电泳迁移率变动分析(EMSA)、DNase I 足迹法(DNase I footprinting)、酵母单杂交技术(Y1H) 以及染色质免疫沉淀技术(ChIP) 的原理、优缺点、优化方法及其最新应用等进行了综述。

Interaction and biological research methods of DNA-protein

SUN Yu-Hang， WANG Yu-Xiang*

(Key Laboratory of Chicken Genetics and Breeding of Ministry of Agriculture， Key Laborabory of Animal Genetics， Breeding and Reproduction of Education Department of Heilongjiang Province， College of Animal Science and Technology， Northeast Agricultural University， Harbin 150030， China)

Abstract: Abstract: DNA and protein are two kinds of most important macromolecules in organisms. Their specific or nonspecific identification and interaction play important roles in the expression and regulation of whole genome， and help to understand the basic processes of life activities. Therefore， in this article， we summarize the concept and types of DNA-protein interaction， and review the principles， merits and demerits， optimization and application of several biologcial detection methods of DNA-protein interactions， such as electrophoretic mobility shift assay (EMSA)， DNA footprinting (DNase I footprinting)， yeast one-hybrid (Y1H) and chromatin immunoprecipitation (ChIP).