《米兰app官网版下载官方版》 2019, 31(5): 423-429

假单胞菌中基于羟基苯甲酸的合成生物学

王松伟，张雪洪*

(上海交通大学米兰app官网版下载官方版技术学院，微生物代谢国家重点实验室，上海 200240)

摘　要：摘　要：假单胞菌是一类重要的环境微生物，具有卓越的物质分解转化和合成能力，利用莽草酸途径可以合成多种芳香族化合物，包括羟基苯甲酸等平台产品。羟基苯甲酸，如2- 羟基苯甲酸(2-HBA)、3- 羟基苯甲酸(3-HBA)、4- 羟基苯甲酸(4-HBA) 和3，4- 二羟基苯甲酸(PCA)，在假单胞菌碳代谢中起着重要作用。以羟基苯甲酸作为合成生物学的一个基本模块，用于合成高附加值的衍生物具有广阔的前景。探究基于不同羟基苯甲酸为前体的细胞工厂建立，可为假单胞菌合成生物学平台的应用和构建打下良好的基础。

Biosynthesis based on hydroxybenzoate acid in Pseudomonas

WANG Song-Wei， ZHANG Xue-Hong*

(State Key Laboratory of Microbial Metabolism， School of Life Sciences and Biotechnology， Shanghai Jiao Tong University， Shanghai 200240， China)

Abstract: Abstract: Pseudomonas has been well engineered as typical microorganisms for value-added aromatic compound production including hydroxybenzoate acid on the basis of shikimate pathway. Hydroxybenzoate acids， such as 2-hydroxybenzoate acid (2-HBA)， 3-hydroxybenzoate acid (3-HBA)， 4-hydroxybenzoate acid (4-HBA) and 3，4-dihydroxybenzoate acid (PCA)， play key roles in the microbial carbon metabolism of Pseudomonas. Using the versatile intermediate platform of hydroxybenzoate acid， a lot of high-value products can be synthesized efficiently. With a close-up at the future perspectives， a series of commercial biological products could be developed through the design and construction of artificial biosynthetic pathways in conventional microorganisms Pseudomonas for synthetic biology platform construction.